TEMPORAL ORDER IN MAMMALIAN CELLS

Temporal Order in Mammalian Cells

Chinese hamster cells were synchronized by the Colcemid-selection system. In cells with a division cycle time of 11.5-12 hr, the activity of the enzyme lactate dehydrogenase (LDH) underwent marked oscillations with a 3.5-hr period. Precipitation of labeled LDH enzyme with specific antibody indicated that the enzyme activity changes were the result of intermittent enzyme synthesis and relatively...

TEMPORAL ORDER IN MAMMALIAN CELLS I. The Periodic Synthesis of Lactate Dehydrogenase in the Cell Cycle

Chinese hamster cells were synchronized by the Colcemid-selection system . In cells with a division cycle time of 11 .5-12 hr, the activity of the enzyme lactate dehydrogenase (LDH) underwent marked oscillations with a 3 .5-hr period . Precipitation of labeled LDH enzyme with specific antibody indicated that the enzyme activity changes were the result of intermittent enzyme synthesis and relati...

Temporal organization of replication in DNA fibers of mammalian cells

The extent of coordinate control over the multiple initiation events in DNA replication has been investigated in three mammalian cell lines by DNA fiber autoradiography. Quantitative estimates have been obtained of the degree of synchrony among initiations occurring on stretches of DNA. Synchrony decreases markedly with increasing distance between initiation sites in MDBK (bovine) and L929 (mou...

The spatial order of transcription in mammalian cells.

We have previously developed technology for multiplexing probes for the detection of transcription of many genes simultaneously within single cells. This has allowed us to determine the spatial localization of multiple genes with respect to each other in the nucleus, and ultimately the expression profile of the cell with respect to surrounding cells in a tissue. Six parameters of transcriptiona...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells